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TitleMicrosatellite identification and characterization in peanut (A. hypogaea L.)
AuthorsM. E. Ferguson, M. D. Burow, S. R. Schulze,
P. J. Bramel, A. H. Paterson, S. Kresovich,
S. Mitchell
PublicationTheor Appl Genet 108:1064-1070
AbstractA major constraint to the application of
biotechnology to the improvement of the allotetraploid
peanut, or groundnut (Arachis hypogaea L.), has been the
paucity of polymorphism among germplasm lines using
biochemical (seed proteins, isozymes) and DNA markers
(RFLPs and RAPDs). Six sequence-tagged microsatellite
(STMS) markers were previously available that revealed
polymorphism in cultivated peanut. Here, we identify and
characterize 110 STMS markers that reveal genetic
variation in a diverse array of 24 peanut landraces. The
simple-sequence repeats (SSRs) were identified with a
probe of two 27,648-clone genomic libraries: one constructed
using PstI and the other using Sau3AI/BamHI.
The most frequent, repeat motifs identified were ATT and
GA, which represented 29% and 28%, respectively, of all
SSRs identified. These were followed by AT, CTT, and
GT. Of the amplifiable primers, 81% of ATT and 70.8%
of GA repeats were polymorphic in the cultivated peanut
test array. The repeat motif AT showed the maximum
number of alleles per locus (5.7). Motifs ATT, GT, and
GA had a mean number of alleles per locus of 4.8, 3.8,
and 3.6, respectively. The high mean number of alleles
per polymorphic locus, combined with their relative
frequency in the genome and amenability to probing,
make ATT and GA the most useful and appropriate motifs
to target to generate further SSR markers for peanut.

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