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TitleLeveraging the rice genome sequence for monocot comparative and translational genomics
AuthorsH. C. Lohithaswa, F. A. Feltus, H. P. Singh, C. D. Bacon, C. D. Bailey, A. H. Paterson
Year2007
Taxonrice
PDFLohith_et_all_2007.pdf
PublicationTheor Appl Genet
Journal_link
Publisher_note
SupplementalAbstract Common genome anchor points across many taxa greatly facilitate
translational and comparative genomics and will improve our understanding of
the Tree of Life. To add to the repertoire of genomic tools applicable to the
study of monocotyledonous plants in general, we aligned Allium and Musa ESTs to
Oryza BAC sequences and identiWed candidate Allium-Oryza and Musa-Oryza
conserved intron-scanning primers (CISPs). A random sampling of 96 CISP primer
pairs, representing loci from 11 of the 12 chromosomes in rice, were tested on
seven members of the order Poales and on representatives of the Arecales,
Asparagales, and Zingiberales monocot orders. The single-copy ampliWcation
success rates of Allium (31.3%), Cynodon (31.4%), Hordeum (30.2%), Musa (37.5%),
Oryza (61.5%),Pennisetum (33.3%), Sorghum (47.9%), Zea (33.3%), Triticum (30.2%)
, and representatives of the palm family (32.3%) suggest that subsets of these
primers will provide DNA markers suitable for comparative and translational
genomics in orphan crops, as well as for applications in conservation biology,
ecology, invasion biology, population biology, systematic biology, and related
Welds.
Abstract

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