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MerMade
Centrifugation
Electrophoresis and blotting
Sequencing
QBOT
Scheduling
(3 periods per day, then late night hours available)
Cleaning
(pins, etc) and maintenance of the room
Supplies
(plates,media, trays, etc)
Repairs
Pipetmen and Tips:
Guru:
Associate
guru:
Pipetmen:
_________
Each Pipetman in the lab is to be identified by a unique number,
and have specific records maintained in a ringbound
notebook kept by the guru. The ‘resume’
for each Pipetman should show its serial number, when
it was purchased, from whom it was purchased (for those we brought from
Each Pipetman is to be assigned to a person or location. ‘Spares’ will be kept by the guru for new
people or for temporary use by others when their assigned pipetman
has a problem. A separate set of spares
of one P10 and one P200 will be kept for the hot room.
Problems
with a Pipetman are to be brought immediately to the
attention of the guru (or in his/her absence the associate guru), who will
promptly provide a ‘temporary pipetman’ and then will
determine and repair the problem.
In
addition to maintaining spare pipetmen, the guru/associate
guru will have responsiblility for keeping on hand a
supply of spare O-rings, seals, shafts, and other parts for the most common pipetman sizes (P20, P200, P1000) so that repairs can be
made quickly. It is expected that these
things will be ordered several at a time, to minimize personnel time, shipping
costs, and take advantage of any other economies of scale that we can.
The Pipetman gurus also oversee supplies of tips, be sure that
we have enough at all times, and oversee the use of specialized (i.e.
expensive) tips that are for special purposes only. When tip racks are low, the Pipatman gurus are at liberty to ‘draft’ student workers to
help replenish tip supplies and see that the racks get sterilized.
In
addition, the gurus are responsible for overseeing our inventories of bulk
tips, and seeing that additional tips are ordered in a timely manner -- assume
that it will take at least 1 month for them to arrive. All bulk tips (to the extent possible) are to
be stored in a location that is close to the tip-loading area (adjacent to
small autoclave in 155), along with peripheral stuff such as tip-loading racks,
etc. The gurus should see that the
locations fo tip-related
items are properly noted on the overall ‘lab inventory’ list (so anyone in the
lab can find them in a pinch).
The gurus
oversee the glass pipet-washing system, maintaining
the system, making sure that glass pipets are
regularly washed and a supply is kept available, and watching for causes of
damage to them (they are expensive!) that need to be discussed in the general
lab meeting.
____________
Hot room (159) and hot room
support area (157 east wall).
Preparations
for 157 hot-room support area.
(a) Move
the non-heated orbital shaker from the hot room to this bench, set it up back
near the sink.
(b) Move hyb buffers to the refrigerator, use the racks in the door,
so people can store their 'in use' blots in the refrigerator itself.
(c) Bring
the Saran wrap in here, keep a lot in a drawer, and a
couple of rolls on the bench.
(d) Move
SSPE and/or SSC carboys in there. Set up
a carboy for 0.1 N NaOH for stripping. Keep a bottle of 20% SDS in there. Establish a place to keep plastic beakers (1,
2, and 4 liter), plastic graduated cylinders (10 ml and 100 ml), and stock it
with them. ASK THE PPD GUYS TO INSTALL A
SINGLE SHELF, WITHIN REACH, OVER THE SINKS TO HOLD THE CARBOYS.
(e) Move
the larger microwave (the old brown one, not the black one) from 155 into
157. Try to find a way to get it up out
of the way (but still in reach), in a similar manner to what I have done for
the black one in 155 (see it).
(f) Set
up the waterbath that I have put in there -- it is a
tall one, and was purchased specifically for heating bottles of hyb buffer. Katy,
please check every couple of days to make sure it does not go dry.
(g) The
prepared fish DNA is in the -20 chest freezer -- perhaps we should move the
additional bottles of unprepared fish DNA there too -- they are presently in
the chromatography fridge in 158. Please
prepare a lot (such as 100 50-ml tubes worth) so we need not do it again for a
long while).
(h)
Rubbermaid boxes and lids for stripping should be moved in here, probably
stored on a low shelf. Scott may be able
to rig up some more wire shelves, as per the QBOT room. ??Is there a place to store ALL hyb containers in here??
(i) I found some more 8x10 intensifying screens in a
container in drawer A11 of Room 157 -- please put these in some of the cassettes
that have no screen -- the screens are NOT to be exposed to light, as it causes
them to fluoresce and results in background on the films -- so use the darkroom
(always!).
(j) Find
out if we have any of the largest alligator clips available (we may have
brought some from Texas) - get more as needed to bring our supply to a minimum
of 200, and keep them near the plexiglass shields as
they will be used together.
(k)
Determine how many of each size (8x10, 10x12) of plexiglass
shield we have (please check the lab organization records to make sure they
have all been found), and tell me. We
may need to have more cut (1/8' thickness).
There also need to be about 40 8 x 10 x 1/8" shields in the gel
room for blotting.