Electrophoresis and blotting
Scheduling (3 periods per day, then late night hours available)
Cleaning (pins, etc) and maintenance of the room
Supplies (plates,media, trays, etc)
Pipetmen and Tips:
Each Pipetman in the lab is to be identified by a unique number,
and have specific records maintained in a ringbound
notebook kept by the guru. The ‘resume’
for each Pipetman should show its serial number, when
it was purchased, from whom it was purchased (for those we brought from
Each Pipetman is to be assigned to a person or location. ‘Spares’ will be kept by the guru for new people or for temporary use by others when their assigned pipetman has a problem. A separate set of spares of one P10 and one P200 will be kept for the hot room.
Problems with a Pipetman are to be brought immediately to the attention of the guru (or in his/her absence the associate guru), who will promptly provide a ‘temporary pipetman’ and then will determine and repair the problem.
In addition to maintaining spare pipetmen, the guru/associate guru will have responsiblility for keeping on hand a supply of spare O-rings, seals, shafts, and other parts for the most common pipetman sizes (P20, P200, P1000) so that repairs can be made quickly. It is expected that these things will be ordered several at a time, to minimize personnel time, shipping costs, and take advantage of any other economies of scale that we can.
The Pipetman gurus also oversee supplies of tips, be sure that we have enough at all times, and oversee the use of specialized (i.e. expensive) tips that are for special purposes only. When tip racks are low, the Pipatman gurus are at liberty to ‘draft’ student workers to help replenish tip supplies and see that the racks get sterilized.
In addition, the gurus are responsible for overseeing our inventories of bulk tips, and seeing that additional tips are ordered in a timely manner -- assume that it will take at least 1 month for them to arrive. All bulk tips (to the extent possible) are to be stored in a location that is close to the tip-loading area (adjacent to small autoclave in 155), along with peripheral stuff such as tip-loading racks, etc. The gurus should see that the locations fo tip-related items are properly noted on the overall ‘lab inventory’ list (so anyone in the lab can find them in a pinch).
The gurus oversee the glass pipet-washing system, maintaining the system, making sure that glass pipets are regularly washed and a supply is kept available, and watching for causes of damage to them (they are expensive!) that need to be discussed in the general lab meeting.
Hot room (159) and hot room support area (157 east wall).
Preparations for 157 hot-room support area.
(a) Move the non-heated orbital shaker from the hot room to this bench, set it up back near the sink.
(b) Move hyb buffers to the refrigerator, use the racks in the door, so people can store their 'in use' blots in the refrigerator itself.
(c) Bring the Saran wrap in here, keep a lot in a drawer, and a couple of rolls on the bench.
(d) Move SSPE and/or SSC carboys in there. Set up a carboy for 0.1 N NaOH for stripping. Keep a bottle of 20% SDS in there. Establish a place to keep plastic beakers (1, 2, and 4 liter), plastic graduated cylinders (10 ml and 100 ml), and stock it with them. ASK THE PPD GUYS TO INSTALL A SINGLE SHELF, WITHIN REACH, OVER THE SINKS TO HOLD THE CARBOYS.
(e) Move the larger microwave (the old brown one, not the black one) from 155 into 157. Try to find a way to get it up out of the way (but still in reach), in a similar manner to what I have done for the black one in 155 (see it).
(f) Set up the waterbath that I have put in there -- it is a tall one, and was purchased specifically for heating bottles of hyb buffer. Katy, please check every couple of days to make sure it does not go dry.
(g) The prepared fish DNA is in the -20 chest freezer -- perhaps we should move the additional bottles of unprepared fish DNA there too -- they are presently in the chromatography fridge in 158. Please prepare a lot (such as 100 50-ml tubes worth) so we need not do it again for a long while).
(h) Rubbermaid boxes and lids for stripping should be moved in here, probably stored on a low shelf. Scott may be able to rig up some more wire shelves, as per the QBOT room. ??Is there a place to store ALL hyb containers in here??
(i) I found some more 8x10 intensifying screens in a container in drawer A11 of Room 157 -- please put these in some of the cassettes that have no screen -- the screens are NOT to be exposed to light, as it causes them to fluoresce and results in background on the films -- so use the darkroom (always!).
(j) Find out if we have any of the largest alligator clips available (we may have brought some from Texas) - get more as needed to bring our supply to a minimum of 200, and keep them near the plexiglass shields as they will be used together.
(k) Determine how many of each size (8x10, 10x12) of plexiglass shield we have (please check the lab organization records to make sure they have all been found), and tell me. We may need to have more cut (1/8' thickness). There also need to be about 40 8 x 10 x 1/8" shields in the gel room for blotting.